The methods currently used to detect acute DENV infections that are endorsed by the World Health Organization are isolation of dengue viral antigens and detection of viral nucleic acid in blood categorized by a positive reverse transcriptase–polymerase chain reaction (RT-PCR), immunoglobulin type M (IgM) seroconversion, and/or a four-fold or greater rise in immunoglobulin G (IgG) antibody titers in paired blood samples collected at least 14 days apart [5,6]. The gene discussed is CD40LG; the disease is dengue disease.