Using whole-exome sequencing, we screened for variants in unrelated patients presenting inherited optic neuropathies (ION) with a negative molecular diagnosis after analyzing OPA1, OPA3, WFS1, SPG7, AFG3L2, DNM1L, MFN2 exonic sequences, and pathogenic variants in the mitochondrial DNA responsible for LHON. This evidence concerns the gene OPA1 and Leber hereditary optic neuropathy.