BRD2 and polycystic ovary syndrome: In this manuscript, we describe a high-throughput LC-MS/MS method for measuring seven ovarian steroid hormones and use this technique to measure how the steroid metabolome is altered by three gonadotropin milieus: (1) our standard FSH-only growth medium, (2) a growth medium with 1:1 hCG:FSH ratio, which mimics normal physiology, and (3) a growth medium with 3:1 hCG:FSH ratio, which has been reported for patients with PCOS.