Taken together, our observations of steroid hormone secretion by ovaries cultured in a PCOS-like 3:1 hCG:FSH growth media showed several features of the PCOS phenotype, including hyperandrogenism; a decreased luteal-to-follicular progesterone ratio, which suggests impaired ovulation, and characteristic differences in enzyme activity, implied by hormone precursor-to-product ratios. This evidence concerns the gene BRD2 and hyperandrogenism.