CD274 and anaplastic large cell lymphoma: FISH studies that assessed PD-L1/2 were performed in 25 ALCL cases: 5 cases showed polyploidy (three to four copies); but there was no evidence of rearrangements, deletions, gains, nor amplification of the PD-L1 locus, suggesting that PD-L1 expression in ALCL may not be related to PD-L1 amplification or rearrangement [108].