In leiomyoma, most of the IGF members analyzed at long-term cultures showed increased expression compared to T0, being IGF1 (3-fold, p < 0.01), IGF1R (5-fold, p < 0.001), IGF2R (4-fold, p < 0.05), IGFBP2 (5-fold, p < 0.001), IGFBP3 (1600-fold, p < 0.001) and IGFBP6 (2-fold, p < 0.05) mRNA significantly upregulated (Figure S4D). Here, IGFBP3 is linked to leiomyoma.