The Western blot analysis results revealed significantly reduced USP6NL expression in the U87MG-R and T98G-R cells (Figure 2C) and notably, reduced EGFR expression (Figure 2C) in both the USP6NL-knockdown U87MG-R and T98G-R cells, demonstrating a positive association between USP6NL and EGFR in TMZ-resistant GBM cells. This evidence concerns the gene EGFR and glioblastoma.