The immunofluorescence intensity of ATG5, ATG7, Beclin-1, and LC3A/B all tended to be induced in both the USP6NL-knockdown and TMZ (200 μM)-treated U87MG-R and T98G-R cells compared with the control scramble (Figure 6B); this result indicates the role of USP6NL in the evasion of cell death and is one of the hallmarks of cancer [36]. This evidence concerns the gene ATG5 and cancer.