The decrease in the blood concentration of TNF-α in pair-fed rats could have been due to a lower expression of this cytokine in other localizations, such as subcutaneous adipose tissue [30], while the increase in insulin sensitivity reduces the phosphorylation of JNK [31] and, in this way, calorie restriction prevents the development of insulin resistance in rat skeletal muscle [32]. The gene discussed is INS; the disease is Insulin resistance.