T-cells from the AML BM microenvironment also express several immune checkpoint molecules such as PD1, CTLA-4, LAG3, TIM3, GITR, OX40, 4-1BB and ICOS, whereas AML blasts express 4-1BBL, CD80, CD86, ICOSL, PD-L1, PD-L2 and OX40L. This evidence concerns the gene ICOS and acute myeloid leukemia.