AKT1 and cancer: Studies reveal that various cancer cell lines grown in 2D and 3D cell cultures show differences in cancer-related pathways like mTOR-AKT-S6K (mechanistic target of rapamycin (mTOR)-ribosomal S6 kinase (S6K) pathway), and also vary in their drug response, thus making it important to switch to 3D models [32,33,34].