CASP3 and neoplasm: Activation of SIRT1 in tumor cells can activate AMPK/SIRT1 signaling at higher intracellular NAD+ concentrations, and further increase the expression of NF-κB (nuclear factor kappa B) to stimulate Bax activation and cytochrome c release, therefore triggering the cleavage of GSDME by caspase-3, which is a typical pyroptosis protein [47,48].