NR1D1 and cancer: To investigate the putative effect of a disrupted circadian clock machinery in cancer-associated properties in human CRC cells, in particular EMT and cell migration, we established knockout (KO) mutants for ARNTL, PER2 or NR1D1 using CRISPR-Cas9 in HCT116 cells, as well as stable knockdowns (KD) of the same genes in SW480 and SW620 cell lines, and investigated both clock- and cancer cell-related phenotypes (Figure 1 and Figure 2; Supplementary Figure S1 and S2).