To assess whether EZH2-dependent gene regulation differs in p53wt and p53mut PDAC, we first filtered for genes which were significantly upregulated upon EZH2 knockdown in KC- and NKC cells (FPKM > 0.01; log2fold change > 0.5; q value < 0.05; KC: 384 genes, NKC: 1322 genes). This evidence concerns the gene CALCA and keratoconus.