CD8A and neoplasm: In our single-cell analysis of non-epithelial subsets along the spatial continuum of normal-appearing lung tissues (tumor-distant to -adjacent) and matching early-stage LUADs (see previous paragraph), we identified changes in immune subsets that evolved with increasing proximity to the tumor and that could signify early alterations in the TME, such as increased signatures and fractions of Tregs, as well as reduced cytotoxic CD8+ T cells, antigen-presenting macrophages, and inflammatory DCs [58].