To assess and estimate the impact of cell–cell communications (either homotypic or heterotypic), we investigated the juxtacrine interaction between CAFs/tumor cells in the context of Notch3 signaling and also whether potential heterogeneity in the CAFs or tumor cells leads to differential levels of Jagged1 ligand that can drive the Notch3 activation differentially in EOC cells and its functional consequence. Here, NOTCH3 is linked to neoplasm.