In HCM and cardiac muscle contraction pathways-related DEGs, the expression levels of Actb, Actg1, Des, Myl2/3, Cox4i1, Cox5a, Cox6a2, Cox7a1 and Tnnc1/i3/t2 were increased, while the expression levels of the genes (Dag1, Myh7, Itga2b, Itgb3, Prkab2, Ryr2, Cacna2d1 and Myl4) that promote cardiomyopathy were decreased in the GCN2iB group. Here, DAG1 is linked to cardiomyopathy.