Consistently, expression of canonical EMT factors ZEB1, Vimentin, Snail and ZO-1 were significantly upregulated, and expression of E-cadherin was markedly downregulated at both the mRNA (Figure 2F,G) and the protein levels (Figure 2H) in GREM1-overexpressing CRC cells. The gene discussed is VIM; the disease is colorectal carcinoma.