Next, we assessed the expression levels of several metabolism-related enzymes in tumor sections from H226 cell-derived tumor that were treated with si-NT or sim/hVDAC1-B and were IHC stained using specific antibodies for glycolytic enzymes, hexokinase 1 (HK-I), lactate dehydrogenase (LDH), the TCA cycle enzyme, citrate synthase (CS), and ATP synthesizing enzyme subunit 5A (Figure 5A,B). The gene discussed is HK1; the disease is neoplasm.