In this study, among the 8 DLBCL patients, p53 protein levels were up-regulated in 2 GCB-DLBCL and 1 ABC-DLBCL primary cells, while down-regulated in the remaining 5 primary cells, which indirectly proved that p53 was mutated in the remaining 5 DLBCL primary cells, which was confirmed by our gene detection results. Here, TP53 is linked to diffuse large B-cell lymphoma.