According to previous researches, estrogen affects neuroexcitability and regulates the occurrence of migraine by affecting neurotransmitters such as 5-hydroxytryptamine (5-HT) [30], γ-aminobutyric acid (GABA) [31], calcitonin-gene related peptide (CGRP) [32], opioids [33], and norepinephrine [34], and these neurotransmitters are primarily distributed in the raphe nuclei pontis, periaqueductal gray (PAG), ventral tegmental area (VTA), hypothalamus, and the ventromedial side of the thalamus, which suggests that MwoA is more closely related to the hypothalamus, thalamus, and brainstem than MwA. The gene discussed is CALCA; the disease is migraine disorder.