Indeed, in the present study we revealed a novel coupling of TRPC1 and the Ca2+ entry mode of NCX1 in GC development because: 1) both NCX1 and TRPC1 play similar oncogenic roles in GC; 2) GC cell proliferation and migration could be further attenuated by a combination of selective blockers and specific knockdown of NCX1 and TRPC1; 3) CaCl2 and Hp virulence factors could stimulate TRPC1 and NCX1 coupling to induce Ca2+ signaling; 4) a protein-protein interaction of TRPC1 and NCX1 is verified in GC cells. This evidence concerns the gene SLC8A1 and gastric cancer.