In order to test whether the reported effect of DJ-1 upon the activity of the cell surface ATP-synthase is dependent on the extracellular form, we tested the extracellular ATP generation in ECs cultures subjected to ischemia or normoxia, in the presence and the absence of extracellular recombinant full-length DJ-1 and DJ-1∆C (at 100 nM). Here, PARK7 is linked to ischemia.