Colony formation ability was inhibited by silencing ATG9B (Figure 2A), and an in vitro limiting dilution assay demonstrated that knockdown of ATG9B impeded the self‐renewal ability of gliomas, which was confirmed by the reduced number and size of tumorspheres derived from glioma cells expressing shATG9B relative to those expressing shNC (Figure 2B,C). Here, ATG9B is linked to glioma.