IFNG and neoplasm: CAR T cells as effectors, NALM-6 tumor cells as targets, and prefunctionalized beads coated with IFN-γ capture antibody as cytokine sensors were loaded sequentially onto a nanowell grid array, and the kinetics of killing and end-point cytokine secretion from the same cells was monitored using TIMING (Figure 1, A and B, and Supplemental Video 1).