CD58 and cancer: Our combined functional, transcriptional, and phenotypic data advance the role of CD2-CD58 interactions at the single-cell level and are complementary to independent cell-cell interaction mechanistic studies probing the genes/proteins essential for cancer immunotherapy using CRISPR-Cas9 screens that mimic loss-of-function mutations involved in resistance to these therapies or in CRISPR-Cas9 screens that identify molecules essential for cytokine secretion (28, 29).