In terms of the preoperative clinicopathological characteristics of the development cohort and validation cohort, except for gross classification (p = 0.016), there were no significant differences in age, sex, BMI, grade of differentiation, EMVI, the distance of the tumor from the anal verge, cT stage, perirectal lymph node status, preoperative CEA level, preoperative CA19-9 level, or largest short-axis diameter of LPLN (Table 1). This evidence concerns the gene CEACAM5 and neoplasm.