To explore the functions of PROS1 in LGG tissues at the single-cell level, CancerSEA was used to explore phenotypes possibly regulated by PROS1. As shown in t-SNE and box plots (Additional file 1: Fig. S10), PROS1 was upregulated at the single-cell level in EXP0059 glioma cell groups. The gene discussed is PROS1; the disease is glioma.