After verifying the tumor expression profiles by the above methods, we concluded that the SK-HEP-1 cell line was GPC3− and CD133−, the HepG2 cell line was GPC3+ and CD133−, the PLC8024 cell line was GPC3+ and CD133−, and the Huh7 cell line was GPC3+ and CD133+. Here, GPC3 is linked to neoplasm.