Specifically, the present study had the following objectives: (1) to investigate in vitro cancer-killing efficacy of TRAIL on HCT-116 cells, (2) to determine the cargo protection ability of mPEG-Coa and sustained cargo release kinetics, and (3) to determine the ability of mPEG-Coa-mediated TRAIL delivery to inhibit tumor recurrence. Here, TNFSF10 is linked to neoplasm.