Thus, these observations suggest that the α2-adrenergic receptor agonist, BRI, has beneficial effects on POAG-related human TM by inhibiting the TGF-β2-induced increases in (1) the barrier functions in the 2D monolayers and (2) the hardness of the 3D spheroids, although these BRI-induced effects were measurably different between the 2D and 3D cultures in the HTM cells. The gene discussed is TGFB2; the disease is open-angle glaucoma.