Therefore, to better understand the role of LDHB in NSCLC tumorigenesis, we developed a preclinical GEMM of NSCLC in which lung tumors can be induced by induction of KRAS mutation and TP53 deletion in addition to LDHB depletion or wild-type status, e.g., LDHB+/+; K-rasLSL−G12D/+; p53fl/fl and LDHB−/−; K-rasLSL−G12D/+; p53fl/fl mice, its genotype was verified by Southern blot (Fig. 7a, Supplementary Fig. S7a). This evidence concerns the gene KRAS and non-small cell lung carcinoma.