These confocal observations of bacterial infection and differentiation were in line correlated with the very low nitrogenase enzyme activity of pollux-1 and cyclops-1 nodules and its complete absence in nin-1 nodules with the ORS278 or ORS285 strain (Supplemental Figures S3, S5, S7, S11, S13, and S15). The gene discussed is NIN; the disease is bacterial infectious disease.