Human astrocytes: 86.5 ± 0.5% (TUJ1+HB9+/DAPI+); 83.7 ± 1.9% (TUJ1+ISL1+/DAPI+); ALS mouse astrocytes: 80.0 ± 2.2% This evidence concerns the gene ISL1 and amyotrophic lateral sclerosis.