To distinguish the efficiency of the synthetic nanoparticles (Epi-Nio and Epi-Nio-HA) for the inhibition of breast cancer, the expression of six different genes (i.e., CASP-3, CASP-9, and MMP-2, MMP-9, Cyc-D, and Cyc-E) involved in cell cycle, metastasis, and apoptotic pathways for 4T1 and SkBr3 breast cancer cell lines were assessed (Fig. 6A and B). This evidence concerns the gene MMP2 and breast cancer.