CSTF2 and hepatocellular carcinoma: Figure 5B shows that knockout of CSTF2 significantly inhibited the cell proliferation in Huh7 and MHCC-97H cells by the CCk8 assay. Next, we studied the colony-forming capacity of HCC cells and found that CSTF2-sgRNA Huh7 cells and MHCC-97H cells formed far fewer colonies than the control vector group under low-density culture conditions (Figure 5C). Transwell assay showed that knockout of CSTF2 dramatically inhibited the migration and invasion of Huh7 cells and MHCC-97H cells (Figure 5D).