Western blots were performed with four HC, four NM patients, four M patients, and four post-therapy (MF) metastatic patients to validate the expression level of LOX5, Rac1, Rac1b, p38α, phospho-p38α (Y-182), LIMK1, phospho-LIMK1 (T-508), cofilin1, and phospho-cofilin1 (S-3) proteins. This evidence concerns the gene LIMK1 and nemaline myopathy.