Besides, it was proved that Granzyme B (GZMB)-CD8+ T cells significantly increase in tumor tissues compared with PBMCs while GZMB+CD8+ T cells showed no significant difference, and the expression of GZMB on CD8+ T cells was observed significantly lower in tumor tissues than in peripheral blood, which may indicate both tumor immune resistance-induced spatial limitation and dysfunction of CD8+ T cells (Figure 2C, and Supplementary Figures S2B, C). Here, CD8A is linked to neoplasm.