Other options used the QuantiFERON-TB Gold In-Tube (QFT-GIT) supernatant in people with and without HIV co-infection, to increase the specificity for active TB diagnosis vs. LTBI by looking at differences in multiple host cytokine and chemokine biomarkers assessing multiple unstimulated cytokine/chemokines (IFN-γ, MIP-1β, and TGF-α) coupled with stimulated cytokines (TGF-α and VEGF) (84, 85). This evidence concerns the gene TGFA and tuberculosis.