In vitro studies using DNA methyltransferase and histone deacetylase inhibitors on two CD1d-negative NSCLC cell lines: A549 and SK-MES-1, showed the induction of CD1d expression and cytotoxicity directed toward them by iNKT cells, making epigenetic manipulation an interesting immunotherapeutic approach against NSCLC. This evidence concerns the gene CD1D and non-small cell lung carcinoma.