LAG-3+TIM-3+ TI-Treg cells are also enriched in CRC tumors compared to healthy colon, with increased TGF-β and IL-10 release, and upregulation of CTLA-4 expression compared to LAG-3−TIM-3− TI-Treg cells. The gene discussed is TGFB1; the disease is colorectal carcinoma.