As a proof-of-principle we first assessed DATE specificity in patient-derived models of ccRCC where CA9 is constitutively expressed, irrespective of oxygen tension, due to VHL loss (RCC243) and in the murine cortical adenocarcinoma renal cell carcinoma cell line (Renca) where we overexpressed CA9. This evidence concerns the gene VHL and hereditary clear cell renal cell carcinoma.