Because CD4+ Th1 cells are characterized by the secretion of IFN-γ, the isolated CD4+ T cells were incubated with B16F10 tumor cells in vitro in the presence of isotype or anti-IFN-γ antibody for 24 hours, and the viability of B16F10 cells was assessed by using CCK-8. Here, IFNG is linked to neoplasm.