In this study, we comprehensively demonstrated that cryo-thermal induced Th1-dominant CD4+ T cells, as principal effector cells, inhibited tumor growth and exhibited multiple antitumor immunologic activities to enhance the cytotoxicity of CD8+ T and NK cells, promote the maturation of APCs and MDSCs, and decrease the levels of Tregs and MDSCs to maintain antitumor immune memory. Here, CD8A is linked to neoplasm.