As the role of CD4+ T cells after cryo-thermal therapy and their function in antitumor immunity are described above, we further determined the antitumor immunity of CD4+ T cells in T-cell-deficient hosts in 4T1 model, a model for the study of late-stage triple negative breast cancer (TNBC) (22). The gene discussed is CD4; the disease is triple-negative breast carcinoma.