Additionally, although the level of CD4+ Th1 cells was decreased after depletion of CD8+ T cells when the cryo-thermal treated mice received tumor rechallenge, the level of Treg and Th17 were not influenced, which suggested that CD8+ T cells did not affect Th1 dominance in Th subsets after cryo-thermal therapy. The gene discussed is CD8A; the disease is neoplasm.