Finally, we detected the expression of the proliferation and EMT-related proteins, including Ki-67, PCNA, E-cadherin, vimentin, N-cadherin, and MCM after the knockdown of the c-MET gene using western blot, and the results showed that c-MET knockdown inhibited the ability of proliferation and EMT of colon cancer cells (P < 0.01, Figure 5(d)). Here, PCNA is linked to malignant colon neoplasm.