To assess the transcriptional response of ABC to NPD1, single-cell RT-PCR was used (Figure 3A) to screen the expression of genes related to inflammasome activation and autophagy, both of which have been widely implicated in the pathogenesis of AMD (Mitter et al., 2014; Celkova et al., 2015). The gene discussed is ABCB6; the disease is age-related macular degeneration.