By using multiple immune deconvolution methods to detect the correlation between TOP2A expression and the infiltration levels of different immune cells, including cancer-associated fibroblasts, Tregs, and macrophages, in available cancers, we found out that TOP2A expression was significantly associated with tumor-immune cell infiltration, indicating that TOP2A expression can reflect the status of tumor-immune cell infiltration. The gene discussed is TOP2A; the disease is cancer.