ANXA5 and infection: Specifically, neutrophils were left in medium alone or pretreated with 30 μM inhibitor prior to infection with LVS, and apoptosis was quantified 4, 10, 18 and 24 hours later using Annexin V-FITC/PI staining and flow cytometry as we described (McCracken et al., 2016; Kinkead et al., 2018).