To determine if continued Ng viability after 24 h Ng pre-infection were needed for the obtained results, we exposed Ng pre-infected HeLa/CtE (106 CFU inocula of all Ng strains) to gentamycin to restrict the duration of Ng growth to the 24 h prior to CtE infection (Figure 1A). The gene discussed is NRGN; the disease is infection.