Thus, mutants of methylases involved in the RdDM pathway (DRM2) and methylation maintenance (CMT3, MET1), and a mutant of DNA demethylase ROS1, showed either reproduction and/or infection parameters, and gall size/GC size severely compromised, but this was not observed in CMT2 during early infection (Figs 5, 6). This evidence concerns the gene ROS1 and infection.