Candidate variables were added into Cox proportional hazards regression models of PFS and OS, including age, gender, EMD, subtype, DS staging, ISS staging, R-ISS staging, LDH level, high-risk cytogenetic abnormalities, anemia, renal insufficiency, 1q21 copy numbers, mutation load, ARID family mutations and TP53 mutations. This evidence concerns the gene TP53 and anemia (phenotype).