To verify HAND2 mRNA expression in CRC, another Chinese CRC cohort 2 was measured, and HAND2 expression decreased greatly were observed in CRC tissues (0.958 ± 1.474) than paired normal tissues (1.836 ± 1.583) (n = 86 pairs, P < 0.001) in Additional file 1: Figure S1. The gene discussed is HAND2; the disease is colorectal carcinoma.