A closer look at the downregulated genes contributing to GO terms such as skin development, epidermis development, KC development, and epithelial cell development reveals the downregulation of genes contributing to extracellular matrix formation, intercellular adhesion, and cell arrangement, including Jup, Evpl, Barx2, Arrdc3, and Gja1 (Figure 4c). This evidence concerns the gene BARX2 and keratoconus.