Additionally, both excitatory (PSD95- and VGlut1-positive puncta) and inhibitory (VGAT- and Gephyrin-positive puncta) synapses were detected, though in low numbers, for both control and MJD organoids, which is also an indication of partial neuronal and organoid maturation. This evidence concerns the gene DLG4 and Spinocerebellar ataxia type 3.