Whether the reduced organoids maturation is caused, or not, by the expression of the mutant ataxin-3 is still to be confirmed, ideally by the replication of this study with organoids generated from other control and MJD iPSC cell lines, reducing the possibility that this observation is a result of the described diversity observed between different iPSC cell lines55, rather than the presence of mutant ataxin-3. This evidence concerns the gene ATXN3 and Machado-Joseph disease.