The presence of inhibitory (colocalization of VGAT- and gephrynin-positive puncta) and excitatory (colocalization of VGlut1- and PSD95-positive puncta) synapses was also assessed by confocal microscopy in control and MJD organoids at day 40. This evidence concerns the gene SLC32A1 and Spinocerebellar ataxia type 3.